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J Fr Ophtalmol. 2002 Jun;25(6):590-3.

[Fungal contamination detection in cornea preservation media].

[Article in French]

Author information

1
Laboratoire de Parasitologie-Mycologie, CHU Jean Minjoz, 25030 Besançon, France.

Abstract

PURPOSE:

Fungi can cause major complications following corneal grafting. In this study we aimed to verify the efficiency of a fungal contamination detection protocol for human corneas in preservation before clinical use.

MATERIALS AND METHODS:

The 12 most frequently found species of fungi responsible for keratitis and endophthalmitis were inoculated to preservation medium used by most French eye banks. A protocol used by several centers, including a daily visual control, was followed in order to check that it showed the presence of all microorganisms, particularly slow-growing filamentous fungi.

RESULTS:

Every species was detected in a time from 2 to 4 days.

CONCLUSION:

This microbiological contamination detection protocol of detection for human corneas in organ culture at +31 degrees C, seems to effectively detect the main agents responsible for fungal contamination.

PMID:
12223945
[Indexed for MEDLINE]

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