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Am Rev Respir Dis. 1977 Jun;115(6):1066-9.

Automatable radiometric detection of growth of Mycobacterium tuberculosis in selective media.

Abstract

The formulation of media for selective, automatable, radiometric detection of growth of Mycobacterium tuberculosis in vitro is described. Palmitic-1 acid labeled with carbon-14 and formic-14C acid were compared as substrate sources of [14C]O2 in media deficient in carbohydrate and containing appropriate antimicrobial agents that are not active against tubercle bacilli. A preliminary clinical laboratory study of a medium containing 4 microCi palmitic-1-14C acid per ml showed that this method might provide the basis for practical laboratory use.

PMID:
122228
DOI:
10.1164/arrd.1977.115.6.1066
[Indexed for MEDLINE]

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