Abstract
We analyzed the role of CD8+ T cells on dendritic cells (DCs). Incubation of monocytes with activated but not resting CD8+ T cells induced their differentiation into DCs with a CD1a+ and CD14- phenotype. Activated CD8+ lymphocytes induced down-regulation of CD14 expression in interleukin (IL)-10-treated macrophages and resulted in the expression of DC-related CD1a and up-regulation of mRNA transcripts for RelB, IL-12p40, MCP-2, MIP-1alpha, and ELC. These DCs were potent stimulatory cells in mixed lymphocyte reaction and induced primary CTL responses. This interaction was independent of a direct cell-to-cell contact. Monoclonal antibodies against IFN-gamma completely abolished the CD1a induction on IL-10-treated antigen-presenting cells. These results demonstrate that CD8+ cells can provide stimulatory signals to antigen-presenting cells to induce their differentiation in DC and to increase their immunostimulatory capacity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigen-Presenting Cells / drug effects
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Antigen-Presenting Cells / immunology*
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Antigen-Presenting Cells / physiology
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CD8-Positive T-Lymphocytes / immunology*
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Cell Communication / immunology*
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Cell Differentiation / immunology
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Chemokines / biosynthesis
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Chemokines / genetics
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Chemokines / immunology
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Dendritic Cells / cytology
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Dendritic Cells / immunology*
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Down-Regulation / immunology
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Gene Expression Regulation / immunology
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Humans
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Interferon-gamma / immunology
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Interleukin-10 / immunology
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Interleukin-10 / pharmacology*
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Interleukin-12 / biosynthesis
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Interleukin-12 / genetics
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Interleukin-12 / immunology
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Interleukin-12 Subunit p40
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Lipopolysaccharide Receptors / immunology
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Lipopolysaccharide Receptors / metabolism
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Lymphocyte Activation / immunology
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Macrophages / cytology
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Macrophages / drug effects
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Macrophages / immunology
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Monocytes / cytology
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Monocytes / immunology*
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Protein Subunits
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / immunology
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Transcription Factor RelB
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Transcription Factors / biosynthesis
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Transcription Factors / genetics
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Transcription Factors / immunology
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Up-Regulation / immunology
Substances
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Chemokines
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Interleukin-12 Subunit p40
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Lipopolysaccharide Receptors
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Protein Subunits
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Proto-Oncogene Proteins
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RELB protein, human
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Transcription Factors
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Interleukin-10
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Transcription Factor RelB
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Interleukin-12
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Interferon-gamma