Localisation of CD25+ cells and MHCII+ cells in lymph nodes draining Mycobacterium avium subsp. paratuberculosis vaccination granuloma and the presence of a systemic immune response

Res Vet Sci. 2002 Aug;73(1):77-85. doi: 10.1016/s0034-5288(02)00071-1.

Abstract

Vaccination of goat kids against paratuberculosis protects against lesions and clinical disease. The systemic cellular response was studied in goat kids 3-9 weeks after vaccination. Peripheral blood cells showed increased interferon-gamma production and expression of interleukin-2 receptor (CD25) after stimulation with Mycobacterium avium subsp. paratuberculosis antigens. The lymph node draining the vaccination granuloma was studied three weeks after vaccination in a parallel group of goat kids. In deep cortex, MHCII+ cells were observed surrounded by CD4+ T-cells, while follicular hypertrophy and hyperplasia were prominent in the subcapsular region and along connective tissue trabecula. Comparison of the local and systemic immune responses revealed an inverse relationship between CD25+ T-cells in the lymph node deep cortex and cells in peripheral blood that up-regulate CD25 upon in vitro stimulation, suggesting that activated and regulatory T-cells in the local lymph node influence the level of circulating antigen-specific T-cells following vaccination against paratuberculosis in goats.

Publication types

  • Clinical Trial

MeSH terms

  • Animals
  • Antigens, Bacterial / immunology
  • Bacterial Vaccines / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression Regulation
  • Goat Diseases / immunology
  • Goat Diseases / microbiology
  • Goat Diseases / pathology
  • Goat Diseases / prevention & control
  • Goats / immunology*
  • Goats / microbiology
  • Granuloma / immunology*
  • Granuloma / microbiology
  • Granuloma / pathology
  • Histocompatibility Antigens Class II / immunology*
  • Interferon-gamma / immunology
  • Interferon-gamma / metabolism
  • Lymph Nodes / cytology
  • Lymph Nodes / immunology*
  • Lymph Nodes / microbiology
  • Male
  • Mycobacterium avium subsp. paratuberculosis / immunology*
  • Mycobacterium avium subsp. paratuberculosis / isolation & purification
  • Paratuberculosis / immunology
  • Paratuberculosis / microbiology
  • Paratuberculosis / pathology
  • Paratuberculosis / prevention & control
  • Receptors, Interleukin-2 / analysis
  • Receptors, Interleukin-2 / immunology*
  • T-Lymphocytes / immunology*

Substances

  • Antigens, Bacterial
  • Bacterial Vaccines
  • Histocompatibility Antigens Class II
  • Receptors, Interleukin-2
  • Interferon-gamma