Abstract
Organisation of the cell nucleus is crucial for the regulation of gene expression but little is known about how nuclei are structured. To address this issue, we designed a genomic screen to identify factors involved in nuclear architecture in Saccharomyces cerevisiae. This screen is based on microscopic monitoring of nuclear pore complexes and nucleolar proteins fused with the green fluorescent protein in a collection of approximately 400 individual deletion mutants. Among the 12 genes identified by this screen, most affect both the nuclear envelope and the nucleolar morphology. Corresponding gene products are localised preferentially to the nucleus or close to the nuclear periphery. Interestingly, these nuclear morphology alterations were associated with chromatin-silencing defects. These genes provide a molecular context to explore the functional link between nuclear architecture and gene silencing.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Nucleolus
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Cell Nucleus*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Deletion
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Gene Expression Regulation, Fungal*
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Gene Silencing*
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Genes, Fungal / genetics*
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Genome, Fungal*
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Nuclear Envelope
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Nuclear Pore
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Nuclear Pore Complex Proteins / genetics
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Nuclear Pore Complex Proteins / metabolism
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Protein Transport
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Ribonucleoproteins, Small Nucleolar*
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Telomere / genetics
Substances
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Fungal Proteins
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NOP1 protein, S cerevisiae
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NUP49 protein, S cerevisiae
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Nuclear Pore Complex Proteins
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Nuclear Proteins
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Recombinant Fusion Proteins
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Ribonucleoproteins, Small Nucleolar
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Saccharomyces cerevisiae Proteins