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Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11872-7. Epub 2002 Aug 22.

Prospective isolation of human clonogenic common myeloid progenitors.

Author information

1
Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA. manz@irb.unisi.ch

Abstract

The hierarchical development from hematopoietic stem cells to mature cells of the hematolymphoid system involves progressive loss of self-renewal capacity, proliferation ability, and lineage potentials. Here we show the prospective isolation of early developmental intermediates, the human clonogenic common myeloid progenitors and their downstream progeny, the granulocyte/macrophage and megakaryocyte/erythrocyte progenitors. All three populations reside in the lineage-negative (lin(-)) CD34(+)CD38(+) fraction of adult bone marrow as well as in cord blood. They are distinguishable by the expression of the IL-3R alpha chain, the receptor of an early-acting hematopoietic cytokine, and CD45RA, an isoform of a phosphotyrosine phosphatase involved in negative regulation of cytokine signaling. Multipotent progenitors, early lymphoid progenitors, and the here-defined myeloid progenitors express distinct profiles of hematopoiesis-affiliated genes. The isolation of highly purified hematopoietic intermediates provides tools to better understand developmental programs underlying normal and leukemic hematopoiesis.

PMID:
12193648
PMCID:
PMC129361
DOI:
10.1073/pnas.172384399
[Indexed for MEDLINE]
Free PMC Article

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