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Curr Cancer Drug Targets. 2001 Nov;1(3):241-7.

D-RNAi (messenger RNA-antisense DNA interference) as a novel defense system against cancer and viral infections.

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1
Epiclone Inc., 731 South Chapel Avenue, Suite F, Alhambra, CA 91801, USA.

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  • Curr Cancer Drug Targets. 2003 Jun;3(3):237.

Abstract

D-RNAi (Messenger RNA-antisense DNA interference), a novel posttranscriptional phenomenon of silencing gene expression by transfection of mRNA-aDNA hybrids, was originally observed in the effects of bcl-2 on phorbol ester-induced apoptosis in human prostate cancer LNCaP cells. This phenomenon was also demonstrated in chicken embryos and a human CD4(+) T cell line, H9. The in vivo transduction of beta-catenin D-RNAi was shown to knock out more than 99% endogenous beta-catenin gene expression, while the in cell transfection of HIV-1 D-RNAi homolog rejected viral gene replication completely. D-RNAi was found to have long-term gene knockout effects resulting from a posttranscriptional gene silencing mechanism that may involve the homologous recombination between intracellular mRNA and the mRNA components of a D-RNAi construct. These findings provide a potential intracellular defense system against cancer and viral infections.

PMID:
12188882
PMCID:
PMC4384701
[Indexed for MEDLINE]
Free PMC Article
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