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J Am Chem Soc. 2002 Aug 28;124(34):9968-9.

New natural product families from an environmental DNA (eDNA) gene cluster.

Author information

1
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853-1301, USA.

Abstract

Uncultured bacteria represent a potentially rich source of new and useful natural products. Studying these natural products requires the development of effective yet straightforward methods to access the small-molecule chemical diversity produced by uncultured bacteria. In this study, DNA extracted directly from soil samples (environmental DNA, eDNA) was used to construct cosmid libraries in Escherichia coli, and these clones were then assayed for the production of antibiosis. A 13 open reading frame (ORF) biosynthetic gene cluster (feeA-M) found in one of the antibacterial active clones, CSLC-2, confers to E. coli the production of two new families of natural products that are derived from long chain N-acyltyrosines. The fee gene cluster and three families of the long chain acyl phenols derived from tyrosine (families 1, 2, and 3) are described.

PMID:
12188643
DOI:
10.1021/ja0268985
[Indexed for MEDLINE]

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