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J Exp Bot. 2002 Sep;53(376):1867-70.

Combined expression of S-VSPalpha in two different organelles enhances its accumulation and total lysine production in leaves of transgenic tobacco plants.

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1
Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50250, Israel.

Abstract

Soybean vegetative storage proteins (S-VSPs) accumulate to high levels in vacuoles of both wild types and heterologous plants. Here it is shown that directing S-VSPalpha to two different organelles-chloroplasts and vacuoles-in a single transgenic plant significantly increased its accumulation. Accumulation of S-VSPalpha in heterologous plants correlated with total soluble lysine. Using this approach with essential amino-acid-rich transgene proteins may lead to a breakthrough in improving plant nutritional quality.

PMID:
12177124
[Indexed for MEDLINE]
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