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Mech Dev. 2002 Jun;114(1-2):171-5.

Differential Pax6 promoter activity and transcript expression during forebrain development.

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1
UCLA Neuroscience IDP and Department of Psychiatry and Biobehavioral Science, UCLA School of Medicine, Los Angeles, CA 90024, USA.

Abstract

Three different Pax6 promoters -- P0, P1, and P alpha -- show differential activity in the developing eye and spinal cord. To examine promoter usage during forebrain development, we performed in situ hybridization and reverse transcription-polymerase chain reaction to detect transcripts initiated from each promoter. Promoter-specific transcripts are expressed within subdomains of total Pax6 expression, but differ from one another in their spatial localization and expression over time. Additionally, we identified a novel P0-initiated transcript and detected a developmentally regulated antisense transcript.

PMID:
12175506
[Indexed for MEDLINE]
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