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Am J Vet Res. 2002 Aug;63(8):1207-11.

Evaluation of bacteriologic culture of pooled fecal samples for detection of Mycobacterium paratuberculosis.

Author information

1
College of Veterinary Medicine, Department of Clinical and Population Sciences, University of Minnesota, St Paul 55108-1004, USA.

Abstract

OBJECTIVES:

To compare sensitivity of several methods of bacteriologic culture of pooled bovine fecal samples for detection of Mycobacterium paratuberculosis and evaluate homogeneity in number of M paratuberculosis in pooled fecal samples.

SAMPLE POPULATION:

Feces from 10 dairy cows that shed M paratuberculosis at various concentrations and 1 dairy cow known to be free of infection with M paratuberculosis.

PROCEDURE:

5 fecal pooling methods, 2 culture methods, and 2 pool sizes were evaluated. Each pooled sample contained 1 infected sample and 4 or 9 uninfected samples.

RESULTS:

Sensitivity of detection of M paratuberculosis was greater with smaller pool size (5 vs 10 samples/pool). Detection sensitivity was also associated with concentration of bacteria in the infected sample. Results indicated that, compared with concurrent bacterial culture of individual infected samples, 37 to 44% of pooled samples with low bacterial concentrations yielded positive culture results and 94% of pooled samples with high bacterial concentrations yielded positive results.

CONCLUSIONS AND CLINICAL RELEVANCE:

Bacteriologic culture of pooled fecal samples may provide a valid and cost-effective method of detecting M paratuberculosis infection in cattle herds.

PMID:
12171178
DOI:
10.2460/ajvr.2002.63.1207
[Indexed for MEDLINE]

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