Format

Send to

Choose Destination
Sex Transm Dis. 2002 Jul;29(7):422-5.

Serologic herpes testing in the real world: validation of new type-specific serologic herpes simplex virus tests in a public health laboratory.

Author information

1
San Francisco Department of Public Health, Sexually Transmitted Diseases Prevention and Control Services and Public Health Laboratory, San Francisco, California 94103, USA.

Abstract

BACKGROUND:

Serologic testing for herpes simplex virus type-2 (HSV-2) is being implemented in sexually transmitted disease (STD) clinics.

GOAL:

To determine the performance characteristics of two HSV-2 type-specific serologic assays in a public health laboratory.

STUDY DESIGN:

Sera stored from a cross-sectional study were tested with the Meridian Diagnostics and Focus Technologies HSV-2 ELISA tests and a type-specific strip immunoblot assay (Chiron Corp.) was used as the reference standard.

RESULTS:

Prevalence of HSV-2 infection in this sample was 44%. Compared to the reference standard, the sensitivity of the Meridian Diagnostics HSV-2 test was 95.5% (95% CI 83.3, 99.2) and specificity was 98.2% (95% CI 89.0, 99.9). The Focus Technologies test yielded 97.7% (95% CI 86.5, 99.9) sensitivity and 94.5% (95% CI 83.9, 98.6) specificity.

CONCLUSIONS:

The performance of these HSV-2 type-specific serologic assays was adequate to support their use in high prevalence populations, such as STD clinic patients.

PMID:
12170133
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wolters Kluwer
Loading ...
Support Center