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FEMS Microbiol Lett. 2002 Aug 6;213(2):205-11.

Evaluation of methods for molecular typing and identification of members of the genus Brevibacterium and other related species.

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1
Centro de Biologia Celular, Campus Universitário de Santiago, Departamento de Biologia, Universidade de Aveiro, Portugal.

Abstract

The genus Brevibacterium includes pleomorphic Gram-positive bacteria with a high mol% G+C content. Species in the genus are difficult to identify by classical methods. The discriminatory power of DNA-based methods is assessed. Strains representing the four well established Brevibacterium species, and other related bacteria, were compared by amplified ribosomal DNA restriction analysis (ARDRA), repetitive-sequence-based PCR (rep-PCR) and ribotyping. Fingerprinting by rep-PCR and ribotyping provided complex genomic profiles with the highest discriminatory potential for molecular typing at the strain level, whereas ARDRA showed differentiation from the genus to the species levels. A high degree of heterogeneity within the genus Brevibacterium is apparent, thus indicating that the taxonomy of the genus should be further studied.

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