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Neuron. 2002 Aug 1;35(3):475-87.

EphB forward signaling controls directional branch extension and arborization required for dorsal-ventral retinotopic mapping.

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1
Molecular Neurobiology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, 92037, La Jolla, CA 92037, USA.

Abstract

We report that EphB receptors direct unique axonal behaviors required for mapping the dorsal-ventral (D-V) retinal axis along the lateral-medial (L-M) axis of the superior colliculus (SC). EphBs are expressed in a D-V gradient, ephrin-B1 in a L-M gradient in SC, and ephrin-B3 at its midline. EphBs and ephrin-Bs are expressed in countergradients in retina and SC. Developmental analyses reveal that retinal axons lack D-V ordering along the L-M axis, but directionally extend branches along it to establish ordered arbors. Directed branch extension is disrupted in EphB2; EphB3-deficient mice resulting in lateral ectopic arbors. Mice with kinase-inactive EphB2 have similar D-V mapping defects indicating that forward signaling dominates over reverse signaling. Our data suggest that branches of EphB expressing axons are attracted medially by ephrin-B1, and provide molecular mechanisms for D-V mapping in visual centers.

PMID:
12165470
DOI:
10.1016/s0896-6273(02)00799-7
[Indexed for MEDLINE]

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