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FEBS Lett. 2002 Aug 14;525(1-3):145-50.

Retardation of the G2-M phase progression on gene disruption of RNA binding protein Sam68 in the DT40 cell line.

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Department of Microbiology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, 570-8506, Osaka, Japan.


Sam68 is an RNA binding protein that is tyrosine-phosphorylated by Src during mitosis and has been postulated to have a role in cell cycle control by modulating RNA metabolism. To elucidate the function of this protein, we isolated a Sam68-deficient DT40 cell line by gene disruption. The Sam68-deficient cells exhibited markedly decreased growth and the growth retardation was due to elongation of the G2-M phase, however, the kinase activity associated with Cdc2 remained unaltered. Our results indicate that Sam68 may play a critical role in G2-M progression in a manner independent of the control of Cyclin/Cdc2 kinase activity.

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