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Annu Rev Microbiol. 2002;56:599-624. Epub 2002 Jan 30.

Impact of genomic technologies on studies of bacterial gene expression.

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1
Department of Stomatology, University of California, San Francisco, 94143, USA. rhodius@itsa.ucsf.edu

Abstract

The ability to simultaneously monitor expression of all genes in any bacterium whose genome has been sequenced has only recently become available. This requires not only careful experimentation but also that voluminous data be organized and interpreted. Here we review the emerging technologies that are impacting the study of bacterial global regulatory mechanisms with a view toward discussing both perceived best practices and the current state of the art. To do this, we concentrate upon examples using Escherichia coli and Bacillus subtilis because prior work in these organisms provides a sound basis for comparison.

[Indexed for MEDLINE]

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