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J Am Chem Soc. 2002 Jul 31;124(30):8770-1.

Reactions of methane monooxygenase intermediate Q with derivatized methanes.

Author information

1
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Abstract

The reactivity of intermediate Q of soluble methane monooxygenase from Methylococcus capsulatus (Bath) with a series of derivatized methanes of general formula CH3-R, where R = CN, NO2, and OH, and their deuterated analogues is described. Despite relatively slow reactions, significant kinetic isotope effects, KIEs, are observed in reactions with acetonitrile and nitromethane; however, none is obtained with methanol. In addition, evidence of a substrate-binding step that occurs prior to substrate oxidation is observed.

PMID:
12137510
DOI:
10.1021/ja0265759
[Indexed for MEDLINE]

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