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Vet Immunol Immunopathol. 2002 Sep 25;88(3-4):111-22.

Identification of monoclonal antibodies that cross-react with cytokines from different animal species.

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Department of Veterinary Microbiology, Immunological Laboratory, The Royal Veterinary and Agricultural University, Stigbojlen 7, DK-1870 Frederiksberg C, Copenhagen, Denmark.


Eleven monoclonal antibodies specific for ovine, bovine and human cytokines were investigated by flow cytometry for cross-reactivities with cytokines produced by peripheral blood mononuclear cells (PBMCs) from sheep, cattle, goat, swine, horse, dog, mink, rabbit and human. Four antibodies specific for IL-4, IL-8, IFN-gamma and TNF-alpha cross-reacted with cytokines from a majority of the species investigated. These antibodies can be applied to flow cytometric studies of cytokine production by PBMCs from several veterinary species. Another five antibodies specific for IL-2, IL-6, GM-CSF and IFN-gamma (two antibodies) cross-reacted weakly and with a variable number of animal species. These antibodies could in certain situations be useful in flow cytometry. In a number of cases the immunological cross-reactivities were confirmed by Western blot analyses. Overall, the results of this study will remedy some of the lack of species-specific anti-cytokine antibodies in veterinary research.

[Indexed for MEDLINE]

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