Format

Send to

Choose Destination
Parasitol Res. 2002 Aug;88(8):754-9. Epub 2002 Apr 30.

Resistance to arsenite modulates expression of beta- and gamma-tubulin and sensitivity to paclitaxel during differentiation of Leishmania donovani.

Author information

1
Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab 160062, India.

Abstract

Differentiation of Leishmania donovani promastigotes into infectious amastigotes is accompanied by differential tubulin gene expression. Tubulin is one of the proposed targets of clinically useful antileishmanial agents and its expression is known to alter due to drug resistance. In this study, beta- and gamma-tubulin expression under various stages of differentiation was measured in an in vitro generated arsenite-resistant L. donovani strain. Results showed higher constitutive expression of beta-tubulin in the arsenite-resistant promastigotes and amastigotes compared with the wild-type. beta-Tubulin expression in the resistant promastigotes increased on paclitaxel treatment. Significant differences in gamma-tubulin expression were observed only between the amastigotes, but not between promastigotes, of wild-type and resistant strains. Paclitaxel did not produce any significant change in the expression profile of gamma-tubulin in either of the strains, neither before nor after differentiation. Data suggest that the beta- and gamma-tubulin expression and the response to paclitaxel is affected due to arsenite resistance.

PMID:
12122434
DOI:
10.1007/s00436-002-0632-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center