A fresh autologous pericardial trileaflet valve conduit was used in a 5-month-old infant for correction of truncus arteriosus. The patient recovered from the operation with satisfactory hemodynamics and post-operative echocardiogram at 3 months showed only mild to moderate pulmonary regurgitation. This technique is a useful alternative for correction in an infant with congenital heart disease who needs a tissue valve conduit. It may be more suitable than an aortic homograft by reason of unavailability of small homograft and limitation of organ donation.