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Cell Motil Cytoskeleton. 2002 Jun;52(2):61-5.

Inducing precocious anaphase in cultured mammalian cells.

Author information

1
University of North Carolina, Department of Biology, 607 Fordham Hall, CB# 3280, Chapel Hill, NC 27599-3280, USA. jccanman@email.unc.edu

Abstract

The spindle checkpoint, which prevents anaphase onset upon spindle damage or incorrect chromosome alignment, presents a problem for experimental analysis of protein function in anaphase and cytokinesis. This is because the functional disruption of many proteins before anaphase onset can activate this checkpoint, preventing anaphase and subsequent cell cycle events. This paper compares new and old methods of overriding the spindle checkpoint in prometaphase mammalian tissue culture cells.

PMID:
12112148
DOI:
10.1002/cm.10032
[Indexed for MEDLINE]

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