Format

Send to

Choose Destination
Cell Calcium. 2002 May;31(5):245-51.

Measuring zinc in living cells. A new generation of sensitive and selective fluorescent probes.

Author information

1
Molecular Probes, Inc., 4849 Pitchford Avenue, Eugene, OR 97478, USA. kyle.gee@probes.com

Abstract

New fluorescent indicators with nanomolar to micromolar affinities for Zn(2+) have been synthesized in wavelengths from UV to the far red. The UV light-excited indicators are ratiometric. The visible wavelength indicators are non-ratiometric and exhibit large and pH-independent fluorescence increases with increasing zinc concentrations, with little to no sensitivity to physiologically relevant Ca(2+) concentrations. Experiments in neuronal and non-neuronal cell cultures show the new indicators to retain their sensitivity to and selectivity for zinc after conversion to cell-permeable forms.

PMID:
12098227
DOI:
10.1016/S0143-4160(02)00053-2
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center