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FEBS Lett. 2002 Jul 3;522(1-3):130-4.

Role of intracellular loops of cannabinoid CB(1) receptor in functional interaction with G(alpha16).

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1
Department of Pharmacology, Physiology and Therapeutics, University of North Dakota, Grand Forks 58202, USA. ho.begoniay@kendle.com

Abstract

The cannabinoid CB(1) but not the CB(2) receptor was demonstrated to couple via G(alpha16) to activate phospholipase C after co-expression in COS7 cells. Chimeric CB(1)/CB(2) receptors were used as a model to study receptor-G(alpha16) interaction. Sequences of the second and third intracellular loops and the carboxy-terminus were substituted from the CB(1) into the CB(2) receptor. Only the triple mutant with all three regions replaced activated phospholipase C to a similar extent as the CB(1) receptor, suggesting that all three intracellular regions are required for interacting with G(alpha16). Several sub-domains within the third intracellular loop were identified for receptor-G(alpha16) interaction.

PMID:
12095632
[Indexed for MEDLINE]
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