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J Synchrotron Radiat. 2002 Jul 1;9(Pt 4):198-201. Epub 2002 Jun 30.

Radiation damage of protein crystals at cryogenic temperatures between 40 K and 150 K.

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Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.


X-ray radiation damage of lysozyme single crystals by an intense monochromatic beam from the Advanced Photon Source is studied at cryogenic temperatures between 40 K and 150 K. The results confirm that primary radiation damage is both linearly dependent on the X-ray dose and independent of temperature. The upper limit for the primary radiation damage observed in our previous study [Teng & Moffat (2000), J. Synchrotron Rad. 7, 313-317] holds over the wider temperature range of this study. The X-ray diffraction quality of the data acquired at 40 K is superior to those at 100 K, apparently due to temperature dependence of secondary and tertiary radiation damage and to reduced thermal motion.

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