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Cancer Cell. 2002 Mar;1(2):169-79.

Bcl-2-dependent modulation of Ca(2+) homeostasis and store-operated channels in prostate cancer cells.

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Laboratoire de Physiologie Cellulaire, INSERM EPI-9938, Universite des Sciences et Technologies de Lille, Batiment SN3, 59655 Villeneuve d'Ascq Cedex, France.


Antiapoptotic oncoprotein Bcl-2 has extramitochondrial actions due to its localization on the endoplasmic reticulum (ER); however, the specific mechanisms of such actions remain unclear. Here we show that Bcl-2 overexpression in LNCaP prostate cancer epithelial cells results in downregulation of store-operated Ca(2+) current by decreasing the number of functional channels and inhibiting ER Ca(2+) uptake through a reduction in the expression of calreticulin and SERCA2b, two key proteins controlling ER Ca(2+) content. Furthermore, we demonstrate that Ca(2+) store depletion by itself is not sufficient to induce apoptosis in Bcl-2 overexpressing cells, and that sustained Ca(2+) entry via activated store-operated channels (SOCs) is required as well. Our data therefore suggest the pivotal role of SOCs in apoptosis and cancer progression.

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