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Curr Microbiol. 2002 Aug;45(2):94-8.

Biosynthesis of the branched-chain amino acids in the cyanobacterium Synechocystis PCC6803: existence of compensatory pathways.

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1
Laboratoire de Chimie Bactérienne-CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.

Abstract

Complementation of an E. coli mutant auxotrophic for the branched-chain amino acids (BCAA)--valine, leucine, and isoleucine--by the ilvG gene ( slr2088) of the cyanobacterium Synechocystis PCC6803 indicates that this gene encodes an active alpha-acetohydroxy acid synthase. Differences of response of the recombinants to the addition of the essential amino acids suggested a lower specificity for the initial reaction of the valine/leucine chain than for the isoleucine one. Inactivation of ilvG in Synechocystis led to a leaky phenotype, suggesting a capacity to compensate the auxotrophies by other processes. This observation is discussed in view of the general difficulty of obtaining auxotrophs in cyanobacteria.

PMID:
12070685
DOI:
10.1007/s00284-001-0097-2
[Indexed for MEDLINE]
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