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Fertil Steril. 2002 Jun;77(6):1209-13.

Importance of the biopsy date in autologous endometrial cocultures for patients with multiple implantation failures.

Author information

1
The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Presbyterian Hospital, Weill Cornell Medical Center, New York, New York 10021, USA. sdspando@mail.med.cornell.edu

Abstract

OBJECTIVE:

To analyze the effectiveness of autologous endometrial coculture by the cycle day of the endometrial biopsy.

DESIGN:

Retrospective study.

SETTING:

University-based IVF center.

PATIENT(S):

Two hundred eight patients with multiple IVF failures.

INTERVENTION(S):

Embryos were split and randomly allocated to growth on autologous endometrial coculture or conventional media.

MAIN OUTCOME MEASURE(S):

Embryo quality and pregnancy outcome.

RESULT(S):

The overall clinical pregnancy rate was 41.8%. Embryos grown on autologous endometrial coculture were of higher quality (more blastomeres and less fragmentation) than embryos grown with conventional media. Early luteal biopsies (<5 days after LH surge) for autologous endometrial coculture did not demonstrate an improvement in embryo quality as compared to the significant improvement demonstrated with later luteal endometrial biopsies (> or =5 days after LH surge). The date of the biopsy was predictive of pregnancy outcome when using autologous endometrial coculture (44.7% [> or =5 days after LH surge] vs. 18.8% [<5 days after LH surge], P=.012).

CONCLUSION(S):

We have demonstrated an improvement in embryo quality when using autologous endometrial coculture. The improvement in embryo quality and higher pregnancy rates were limited to biopsies > or =5 days after the LH surge. This suggests that mid/late luteal phase endometrium contains factors that enhanced embryo growth and subsequent implantation.

PMID:
12057730
[Indexed for MEDLINE]
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