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Methods. 2002 Apr;26(4):307-16.

In vivo and in organello assessment of OXPHOS activities.

Author information

1
Department of Biological Sciences, Columbia University, New York, NY 10027, USA. ab775@columbia.edu

Abstract

Mitochondrial OXPHOS defects are responsible for a large group of human diseases and have been associated with degenerative disorders and aging. The accurate in vivo and in organello biochemical assessment of the OXPHOS system is necessary for the diagnosis and investigation of such conditions. Here I describe a set of accurate polarographic and spectrophotometric assays that use relatively small amounts of biological material (cells or isolated mitochondria) and discuss the biochemical parameters appropriate for discriminating partial OXPHOS defects.

PMID:
12054921
DOI:
10.1016/S1046-2023(02)00036-1
[Indexed for MEDLINE]

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