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Biomed Environ Sci. 2002 Mar;15(1):36-40.

Yeast one-hybrid system used to identify the binding proteins for rat glutathione S-transferase P enhancer I.

Author information

1
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China.

Abstract

OBJECTIVE:

To detect the trans-factors specifically binding to the strong enhancer element (GPEI) in the upstream of rat glutathione S-transferase P (GST-P) gene.

METHODS:

Yeast one-hybrid system was used to screen rat lung MATCHMAKER cDNA library to identify potential trans-factors that can interact with core sequence of GPEI(cGPEI). Electrophoresis mobility shift assay (EMSA) was used to analyze the binding of transfactors to cGPEI.

RESULTS:

cDNA fragments coding for the C-terminal part of the transcription factor c-Jun and rat adenine nucleotide translocator (ANT) were isolated. The binding of c-Jun and ANT to GPEI core sequence were confirmed.

CONCLUSIONS:

Rat c-jun transcriptional factor and ANT may interact with cGPEI. They could play an important role in the induced expression of GST-P gene.

PMID:
12046546
[Indexed for MEDLINE]

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