Format

Send to

Choose Destination
Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):1042-4. Epub 2002 May 29.

Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA.

Author information

1
Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Viikinkaari 1, PO Box 65, FIN-00014 University of Helsinki, Finland.

Abstract

A recombinant form of a collagen-binding fragment of Yersinia enterocolitica serotype O:3 adhesin YadA with an N-terminal polyhistidine affinity tag has been produced in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion technique. Crystals belong to the trigonal space group R3, with unit-cell parameters a = b = 67.05, c = 221.95 A, and diffract to 1.55 A resolution on a synchrotron-radiation source.

PMID:
12037311
DOI:
10.1107/s0907444902005231
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for International Union of Crystallography
Loading ...
Support Center