Format

Send to

Choose Destination
J Assist Reprod Genet. 2002 Apr;19(4):183-94.

Noncontact, laser-mediated extraction of polar bodies for prefertilization genetic diagnosis.

Author information

1
1st Women's Hospital, Ludwig-Maximilians-University, Munich, Germany. a-s@sengewald.m.uunet.de

Abstract

PURPOSE:

We tested an entirely noncontact polar body-extraction method using an ultraviolet laser beam for laser zona drilling and a near infrared laser beam for polar body (PB) trapping and extraction.

METHODS:

A hole was drilled into the zona pellucida of an oocyte. Then, the PB was trapped with optical tweezers and dragged through the drilled hole.

RESULTS:

Bovine first PBs could be extracted in 49 out of 63 oocytes (78%) using this method. In human oocytes, PB extraction was successfully demonstrated, which however was more time consuming. A number of extracted PBs were dried on a special membrane, circumcised with the laser microbeam, and successfully catapulted into the lid of a microfuge tube (laser pressure catapulting).

CONCLUSIONS:

This solely laser-mediated extraction method allows convenient procurement of PBs without the danger of contamination and is a promising approach that might replace standard micromanipulation methods in the future.

PMID:
12036086
PMCID:
PMC3455650
DOI:
10.1023/a:1014894029099
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center