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EMBO Rep. 2002 Jun;3(6):551-6. Epub 2002 May 24.

Initial activation of cyclin-B1-cdc2 kinase requires phosphorylation of cyclin B1.

Author information

1
Centre de Recherches de Biochimie Macromoléculaire, CNRS UPR 1086, 1919 route de Mende, F-34293 Montpellier cedex 5, France.

Abstract

At the G(2)/M transition of the cell cycle, the cdc25c phosphatase dephosphorylates inhibitory residues of cdc2, and cyclin-B-cdc2 kinase (MPF) is activated. Phosphorylation of cyclin B1 induces its nuclear accumulation, and, since cdc25c is also believed to accumulate and activate shortly before G(2)/M in the nucleus, it has been proposed that this induces cyclin-B1-cdc2 kinase activation. We demonstrate that cyclin B1 phosphorylation has another essential function in vivo: it is required for cdc25c and MPF activation, which does not require nuclear accumulation of cyclin B1, and occurs in the cytoplasm.

PMID:
12034754
PMCID:
PMC1084145
DOI:
10.1093/embo-reports/kvf111
[Indexed for MEDLINE]
Free PMC Article

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