Format

Send to

Choose Destination
See comment in PubMed Commons below
Biophys J. 2002 Jun;82(6):2928-33.

The role of proofreading in signal transduction specificity.

Author information

1
Center for Studies in Physics and Biology, The Rockefeller University, New York, New York 10021, USA. swain@papagena.rockefeller.edu

Abstract

Many intracellular signaling proteins such as MAP kinases and transcription factors require multiple covalent modifications before activating downstream targets. This property suggests that signaling pathways are organized to facilitate proofreading, which expends energy to enhance the specificity of the pathway for the appropriate effector. Focusing on MAP kinases, we show that each phosphorylation of the kinase can act as an independent specificity test for that kinase. This is independent of whether MAP kinase activation is distributive, processive, or confined to a protein scaffold. We also highlight the importance of phosphatases in developing and maintaining specificity. Support for our proposals can be drawn from the existing literature.

PMID:
12023215
PMCID:
PMC1302080
DOI:
10.1016/S0006-3495(02)75633-6
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center