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Science. 2002 Jun 7;296(5574):1869-73. Epub 2002 May 16.

Two-photon imaging of lymphocyte motility and antigen response in intact lymph node.

Author information

1
Department of Physiology and Biophysics, University of California, Irvine, CA 92697-4561, USA.

Abstract

Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. Visualization of these processes has previously been limited to in vitro systems. We describe the use of two-photon laser microscopy to image the dynamic behavior of individual living lymphocytes deep within intact lymph nodes. In their native environment, T cells achieved peak velocities of more than 25 micrometers per minute, displaying a motility coefficient that is five to six times that of B cells. Antigenic challenge changed T cell trajectories from random walks to "swarms" and stable clusters. Real-time two-photon imaging reveals lymphocyte behaviors that are fundamental to the initiation of the immune response.

PMID:
12016203
DOI:
10.1126/science.1070051
[Indexed for MEDLINE]
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