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Bioelectrochemistry. 2002 May 15;56(1-2):127-9.

Monitoring of ethanol during fermentation using a microbial biosensor with enhanced selectivity.

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Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-842 38 Bratislava, Slovak Republic.


The present study is concerning the construction of ferricyanide-mediated Gluconobacter oxydans cell ethanol biosensor. The size exclusion effect of a cellulose acetate membrane was used for elimination of glucose interferences during ethanol assays in real samples. A typical response time of the biosensor was 13 s with a high sensitivity of 3.5 microA mM(-1). The microbial biosensor exhibits a very low detection limit of 0.85 microM and a wide linear range from 2 to 270 microM. The operational stability was excellent. During 8.5 h of repetitive ethanol assays, no decrease in the sensor sensitivity was observed. The biosensor was successfully used in the off-line monitoring of ethanol fermentation with a good agreement with HPLC measurements (R(2)=0.998).

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