Immunohistochemistry with antibodies to parkin, αS, and UbcH7 in sections of the central and peripheral nervous system. Brainstem Lewy bodies (LB, arrows) are detected by HP2A (a and b), HP1A (c and d), HP7A (e and f), LB509 (g and h), and UbcH7 (t) in substantia nigra sections from PD (a, c, e, g, l, and t) and DLB (b, d, f, h, and m) but not control brain (i, j, and k). Open arrowhead depicts granular staining of perikaryon in a dopaminergic neuron (i); cells of cranial nerve III (j) and neurites (arrowheads) in control nigra (k) stained by HP1A. Competition with respective antigen [HP2A abs. (l) and HP1A abs. (m)] abolishes LB staining but not the appearance of surrounding melanin granules. In entorhinal (n and o) and cingulate cortex (p) sections from DLB brain (n, o, and p), cortical LBs are detected by HP2A (n), HP1A (o), and LB509 (p). In sympathetic gangliocytes from PD (q, r, and s), peripheral LBs are stained by HP2A (q), HP1A (r), and LB509 (s). Scale bar, 20 μm.