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J Microsc. 2002 Apr;206(Pt 1):33-40.

Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object.

Author information

1
Commonwealth Scientific and Industrial Research Organization, Manufacturing Science and Technology, PB 33 Clayton Sth, 3169 Victoria, Australia School of Physics, The University of Melbourne, 3010 Victoria, Australia. David.Paganin@csiro.au

Abstract

We demonstrate simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object. Subject to the assumptions explicitly stated in the derivation, the algorithm solves the twin-image problem of in-line holography and is capable of analysing data obtained using X-ray microscopy, electron microscopy, neutron microscopy or visible-light microscopy, especially as they relate to defocus and point projection methods. Our simple, robust, non-iterative and computationally efficient method is applied to data obtained using an X-ray phase contrast ultramicroscope.

PMID:
12000561
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