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J Immunol Methods. 2002 Apr 1;262(1-2):5-19.

Electrospray mass spectrometry for the identification of MHC class I-associated peptides expressed on cancer cells.

Author information

1
Department of Life Sciences, The Nottingham Trent University, Clifton Lane, NG11 8NS, UK. philip.bonner@ntu.ac.uk

Abstract

Electrospray ionisation (ESI) mass spectrometry (MS) has been used extensively for the detection of peptides presented by major histocompatibility complex (MHC) molecules. This review focuses on the optimisation of electrospray mass spectrometry and the use of tandem mass spectrometry to sequence MHC class I peptides. We review the isolation of MHC class I peptides from the surface of cells with particular reference to tumour cells. In addition, we also discuss the advantages and disadvantages of the methods available to concentrate and fractionate the peptides prior to analysis by electrospray mass spectrometry.

PMID:
11983215
DOI:
10.1016/s0022-1759(02)00009-1
[Indexed for MEDLINE]

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