Format

Send to

Choose Destination
Dev Cell. 2002 Apr;2(4):463-76.

CENP-I is essential for centromere function in vertebrate cells.

Author information

1
PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, 411-8540, Shizuoka, Japan.

Abstract

We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.

PMID:
11970896
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center