Abstract
Glucokinase (GK) is a key enzyme for glucose utilization in liver and shows a higher expression in the perivenous zone. In primary rat hepatocytes, the GK gene expression was activated by HNF (hepatic nuclear factor)-4alpha via the sequence -52/-39 of the GK promoter. Venous pO2 enhanced HNF-4 levels and HNF-4 binding to the GK-HNF-4 element. Thus, HNF-4alpha could play the role of a regulator for zonated GK expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites / genetics
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Cells, Cultured
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DNA-Binding Proteins*
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Gene Expression Regulation, Enzymologic
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Glucokinase / genetics*
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Glucokinase / metabolism*
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Hepatocyte Nuclear Factor 4
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Hepatocytes / metabolism
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Oxygen / metabolism
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Trans-Activators / genetics
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Trans-Activators / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transfection
Substances
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DNA-Binding Proteins
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Hepatocyte Nuclear Factor 4
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Phosphoproteins
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RNA, Messenger
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Recombinant Proteins
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Trans-Activators
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Transcription Factors
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Glucokinase
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Oxygen