Activation of glucokinase gene expression by hepatic nuclear factor 4alpha in primary hepatocytes

Biochem J. 2002 Jul 1;365(Pt 1):223-8. doi: 10.1042/BJ20020340.

Abstract

Glucokinase (GK) is a key enzyme for glucose utilization in liver and shows a higher expression in the perivenous zone. In primary rat hepatocytes, the GK gene expression was activated by HNF (hepatic nuclear factor)-4alpha via the sequence -52/-39 of the GK promoter. Venous pO2 enhanced HNF-4 levels and HNF-4 binding to the GK-HNF-4 element. Thus, HNF-4alpha could play the role of a regulator for zonated GK expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • Cells, Cultured
  • DNA-Binding Proteins*
  • Gene Expression Regulation, Enzymologic
  • Glucokinase / genetics*
  • Glucokinase / metabolism*
  • Hepatocyte Nuclear Factor 4
  • Hepatocytes / metabolism
  • Oxygen / metabolism
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Trans-Activators / genetics
  • Trans-Activators / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transfection

Substances

  • DNA-Binding Proteins
  • Hepatocyte Nuclear Factor 4
  • Phosphoproteins
  • RNA, Messenger
  • Recombinant Proteins
  • Trans-Activators
  • Transcription Factors
  • Glucokinase
  • Oxygen