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FEBS Lett. 2002 Mar 27;515(1-3):20-4.

Arabidopsis glutathione-dependent formaldehyde dehydrogenase is an S-nitrosoglutathione reductase.

Author information

1
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, 739-8526, Higashi-Hiroshima, Japan. ahkkao@hiroshima-u.ac.jp

Abstract

S-Nitrosoglutathione (GSNO), an adduct of nitric oxide (NO) with glutathione, is known as a biological NO reservoir. Heterologous expression in Escherichia coli of a cDNA encoding a glutathione-dependent formaldehyde dehydrogenase of Arabidopsis thaliana showed that the recombinant protein reduces GSNO. The identity of the cDNA was further confirmed by functional complementation of the hypersensitivity to GSNO of a yeast mutant with impaired GSNO metabolism. This is the first demonstration of a plant GSNO reductase, suggesting that plants possess the enzymatic pathway that modulates the bioactivity and toxicity of NO.

PMID:
11943187
DOI:
10.1016/s0014-5793(02)02414-6
[Indexed for MEDLINE]
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