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Arch Dermatol. 2002 Apr;138(4):475-8.

Release and activation of matrix metalloproteinase-9 during in vitro mechanical compression in hypertrophic scars.

Author information

1
Human Anatomy Laboratory, Medical Sciences Department, University of Eastern Piedmont A. Avogadro, Via Solaroli 17, 28100 Novara, Italy.

Abstract

OBJECTIVE:

To investigate induction of matrix metalloproteinases (MMPs) during mechanical compression of hypertrophic scars. Mechanical pressure blocks hypertrophy inducted on extracellular matrix in scars by a mechanism that involves MMP-2 (gelatinase A) and MMP-9 (gelatinase B).

DESIGN:

We assayed conditioned media obtained from normotrophic and hypertrophic scars during 24 hours of in vitro mechanical compression using gelatin zymography.

SETTING:

Scars from various areas of the bodies of hospitalized patients.

PATIENTS:

We obtained 3 normotrophic and 7 hypertrophic biopsy specimens from 10 patients (5 men and 5 women).

INTERVENTION:

In vitro compression at a pressure of 35 mm Hg/cm(2) for 24 hours.

MAIN OUTCOME MEASURES:

Vitality of scars was analyzed by means of lactic dehydrogenase test; medium samples were collected for zymographic analysis of MMP activity.

RESULTS:

We found MMP-2 in basal (uncompressed) samples from normotrophic and hypertrophic scars. Mechanical compression induced MMP-9 release and activation (range, 86.7%-78.7%) in hypertrophic scars after 4 hours.

CONCLUSION:

Production, release, and activation of MMP-9 in hypertrophic scars could be an effector mechanism responsible for hypertrophy regression induced by mechanical compression.

PMID:
11939809
[Indexed for MEDLINE]

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