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J Exp Med. 2002 Apr 1;195(7):919-29.

Major T cell progenitor activity in bone marrow-derived spleen colonies.

Author information

1
INSERM U345, Institut Necker. CNRS UMR 8603, Hôpital Necker. IFR 94 (Institut Fédératif de Recherche Necker-Enfants-Malades), 75730 Paris Cedex 15, France.

Abstract

Common lymphoid progenitors (CLP) are generated in adult bone marrow (BM), but the intermediate steps leading to T cell commitment are unknown, and so is the site at which this commitment occurs. Here, we show that colonies arising in the spleen 12 days after BM injection harbor T cell precursors that are undetectable in BM. These precursors did not generate myeloid cells in vivo but repopulated the thymus and the peripheral T cell compartment much faster than did CLP. Two lineage negative (Lin(-)) subpopulations were distinguished, namely CD44(+) Thy1(-) cells still capable of natural killer generation and transient low-level B cell generation, and T cell-restricted CD44(-) Thy1(+) cells. At a molecular level, frequency of CD3epsilon and preTalpha mRNA was very different in each subset. Furthermore, only the CD44(-) Thy1(+) subset have initiated rearrangements in the T cell receptor beta locus. Thus, this study identifies extramedullary T cell progenitors and will allow easy approach to T cell commitment studies.

PMID:
11927635
PMCID:
PMC2193723
[Indexed for MEDLINE]
Free PMC Article

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