The prolyl oligopeptidase family

Cell Mol Life Sci. 2002 Feb;59(2):349-62. doi: 10.1007/s00018-002-8427-5.

Abstract

A group of serine peptidases, the prolyl oligopeptidase family, cannot hydrolyze peptides containing more than about 30 residues. This group is unrelated to the classical trypsin and subtilisin families, and includes dipeptidyl peptidase IV, acylaminoacyl peptidase and oligopeptidase B, in addition to the prototype prolyl oligopeptidase. The recent crystal structure determination of prolyl oligopeptidase (80 kDa) has shown that the enzyme contains a peptidase domain with an alpha/beta hydrolase fold, and its catalytic triad is covered by the central tunnel of an unusual seven-bladed beta-propeller. This domain operates as a gating filter, excluding large, structured peptides from the active site. The binding mode of substrates and the catalytic mechanism differ from that of the classical serine peptidases in several features. The members of the family are important targets of drug design. Prolyl oligopeptidase is involved in amnesia, depression and blood pressure control, dipeptidyl peptidase IV in type 2 diabetes and oligopeptidase B in trypanosomiasis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Binding Sites
  • Catalysis
  • Dipeptidyl Peptidase 4 / chemistry
  • Dipeptidyl Peptidase 4 / metabolism
  • Humans
  • Kinetics
  • Models, Molecular
  • Oligopeptides / metabolism
  • Peptide Hydrolases / chemistry
  • Peptide Hydrolases / metabolism
  • Prolyl Oligopeptidases
  • Protein Structure, Tertiary
  • Serine Endopeptidases / chemistry*
  • Serine Endopeptidases / metabolism*

Substances

  • Oligopeptides
  • Peptide Hydrolases
  • Dipeptidyl Peptidase 4
  • acylaminoacyl-peptidase
  • Serine Endopeptidases
  • PREPL protein, human
  • Prolyl Oligopeptidases
  • oligopeptidase B