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J Bacteriol. 2002 Apr;184(8):2148-54.

Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.

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  • 1Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118, USA.


Expression of the two Bacillus subtilis genes encoding L-asparaginase is controlled by independent regulatory factors. The ansZ gene (formerly yccC) was shown by mutational analysis to encode a functional L-asparaginase, the expression of which is activated during nitrogen-limited growth by the TnrA transcription factor. Gel mobility shift and DNase I footprinting experiments indicate that TnrA regulates ansZ expression by binding to a DNA site located upstream of the ansZ promoter. The expression of the ansA gene, which encodes the second L-asparaginase, was found to be induced by asparagine. The ansA repressor, AnsR, was shown to negatively regulate its own expression.

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