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EMBO J. 1983;2(3):339-44.

Adenovirus cores can function as templates in in vitro DNA replication.

Author information

1
Division of Virology, National Institute for Medical Research, London, UK.

Abstract

Adenovirus cores prepared by gentle disruption of virus by heating at 56 degrees C in the presence of deoxycholate were able to function as templates in an in vitro DNA replication system, allowing both initiation, indicated by the formation of terminal protein-dCMP complex, and elongation of > 300 nucleotides. Using both cores and DNA-protein complexes as templates, it was also demonstrated that novobiocin, an inhibitor of DNA gyrase, inhibited in vitro DNA replication by preventing formation of the initiation complex.

PMID:
11894947
PMCID:
PMC555138
[Indexed for MEDLINE]
Free PMC Article

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