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Cell. 2002 Mar 8;108(5):717-25.

Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.

Author information

1
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA. rjkuhn@bragg.bio.purdue.edu

Abstract

The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map. The virus core, within a lipid bilayer, has a less-ordered structure than the external, icosahedral scaffold of 90 glycoprotein E dimers. The three E monomers per icosahedral asymmetric unit do not have quasiequivalent symmetric environments. Difference maps indicate the location of the small membrane protein M relative to the overlaying scaffold of E dimers. The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane.

PMID:
11893341
PMCID:
PMC4152842
[Indexed for MEDLINE]
Free PMC Article

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