(A) Surface-shaded representation of dengue-2 cryoEM reconstruction at 24 Å resolution, showing the outline of one icosahedral asymmetric unit and the definition of the coordinate system. Scale bar represents 100 Å.
(B) Central crosssection showing the cryo-EM density with a plot of the maximum (blue) and averaged (purple) density. Arrows indicate the position of the 5-fold and 3-fold axes. Shown also are radial density sections at the defined radii, r1, r2, r3, and r4. Higher density representing protein is shown in dark shading. Scale bar represents 175 Å.
(C) Ribbon drawing of the E dimer situated on an icosahedral 2-fold axis, showing the largest uninterpreted electron density peak outside the lipid bilayer, probably representing the M protein (light blue), located close to the hole between the E dimers. The white arrow indicates the position of the dimer holes. The outer leaflet of the lipid bilayer is shown in green. The domains I, II, and III of an E monomer are shown in red, yellow, and blue, respectively. The fusion peptides are in green.
(D) Ribbon drawing showing the position and orientation of the E dimer associated with an icosahedral 2-fold axis. Shown in white is the outline of one icosahedral asymmetric unit. The domains of E are colored as in (C). The portion of the membrane protein M below the dimer is indicated.