Objective: To select effective methods for accelerating corneal epithelial wound healing and treating persistent corneal epithelial defect.
Methods: Exogenous keratinocyte growth factor (KGF, 0.1 ng/ml - 100 ng/ml) was added to cultures of human corneal epithelial cells and DNA synthesis in corneal epithelial cells was measured with [(3)H]-thymidine incorporation and liquid scintillation techniques. The effective concentration of topical application of KGF was calculated and 1 microg/ml KGF was then applied topically to rabbit eyes. Corneal epithelial defect was measured by a digital computer at different times.
Results: KGF at a dosage ranging from 1 ng/ml to 100 ng/ml enhanced cellular proliferation effectively (the rate of cell proliferation 27.66% - 76.73%) and in a dose-dependent manner (r = 0.9233, P < 0.001). Topical application of KGF significantly accelerated corneal wound healing in rabbits (Epithelial healing rate in the KGF-treated group was 1.77 +/- 0.23 mm(2)/h, while in the PBS-treated group it was 1.49 +/- 0.24 mm(2)/h; P < 0.05).
Conclusion: The results suggest that exogenous KGF stimulate proliferation of human corneal epithelial cells and accelerate corneal epithelial wound healing in vivo.