Format

Send to

Choose Destination
Lett Appl Microbiol. 2002;34(3):162-7.

Development of a high-volume aerosol collection system for the identification of air-borne micro-organisms.

Author information

1
Lawrence Livermore National Laboratory, Livermore, CA 94551, USA.

Abstract

AIMS:

A high-volume aerosol collector was developed to efficiently capture airborne bacteria in order to assess levels of diversity in the air.

METHODS AND RESULTS:

Particulate matter was collected on a device designed to filter 1.4 x 10(6) litres of air in a 24 h period on a 1-microm pore size polyester membrane. Methods were optimized for extraction of genomic DNA from the air filter concentrate. Preparation times of 90 s with 0.5-0. 05 mm diameter zirconia/silica beads yielded the highest concentration genomic DNA that was able to support PCR. A 24-h air sample was taken in Salt Lake City, Utah and the microbial composition was determined by the amplification and sequence analysis of 16S ribosomal DNA fragments.

CONCLUSIONS:

Sequence analysis revealed a large diversity in the type of microbial species present including clones matching the sequence of Clostridium botulinum. The primary components of the aerosol sample included many different spore-forming bacteria as well as more fragile members of the Proteobacteria division.

SIGNIFICANCE AND IMPACT OF STUDY:

The high-volume air collection and genomic DNA recovery system allows for the rapid detection of both cultivable as well as culture-resistant organisms in the environment.

PMID:
11874535
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center