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EMBO J. 2002 Mar 1;21(5):1101-11.

Hyperacetylation of chromatin at the ADH2 promoter allows Adr1 to bind in repressed conditions.

Author information

1
Fondazione Istituto Pasteur-Fondazione Cenci Bolognetti and Centro di Studio per gli Acidi Nucleici, CNR, c/o Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, I-00185 Rome, Italy.

Abstract

We report that in vivo increased acetylation of the repressed Saccharomyces cerevisiae ADH2 promoter chromatin, as obtained by disrupting the genes for the two deacetylases HDA1 and RPD3, destabilizes the structure of the TATA box-containing nucleosome. This acetylation-dependent chromatin remodeling is not sufficient to allow the binding of the TATA box-binding protein, but facilitates the recruitment of the transcriptional activator Adr1 and induces faster kinetics of mRNA accumulation when the cells are shifted to derepressing conditions.

PMID:
11867538
PMCID:
PMC125900
DOI:
10.1093/emboj/21.5.1101
[Indexed for MEDLINE]
Free PMC Article

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